Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
نویسندگان
چکیده
منابع مشابه
Detection of Treponema pallidum by a sensitive reverse transcriptase PCR.
Syphilis is diagnosed by serologic testing or by identification of the causative agent, Treponema pallidum. The bacterium has historically been detected in clinical specimens by dark-field microscopy, immunostaining with polyclonal or monoclonal antibodies, or the rabbit inoculation test (RIT). RIT is considered to be very sensitive and specific, although it is available only in research settin...
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Coliphage MS2 is used in place of pathogens in many studies and is considered one of the indicators of pathogenetic viruses in wastewater. We developed a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay to quantify MS2 coliphages in treated wastewater samples. The format used was SYBR Green. The assay included an internal control to disclose the presence of PCR-produ...
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BACKGROUND Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. OBJECTIVES To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR) for human enterovirus ribonucleic acid (RNA) (QPCR), with protection against amplimer contamination. STUDY DESIGN The method was evaluated with serial dilut...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1997
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.35.6.1348-1352.1997